He can also be found doing crossword puzzles at odd hours. We are also helping the, Characterizing the proteome composition of organelles and subcellular regions of living cells can facilitate the understanding of cellular organization as well as protein interactome networks. Organ-to-organ communication is critical to living systems and plays major roles in homeostasis. Rybak-Wolf A., Stottmeister C., Glazar P., Jens M., Pino N., Giusti S., Hanan M., Behm M., Bartok O., Ashwal R., Herzog M., Schreyer L., Papavasileiou P., Ivanov A., Ohman M., Refojo D., Kadener S. and Rajewsky N. Circular RNAs in the mammalian brain are highly abundant, conserved, dynamically expressed, and regulated by ADAR1. Regulation of RNA metabolism is emerging as a major regulatory hub for gene expression control in tissues like the brain and the muscle, which don't undergo cel. Fun fact:Riley chose her English name when she moved to the U.S because it means courage. Litovchenko M, Meireles-Filho ACA, Frochaux MV, Bevers RPJ, Prunotto A, Anduaga AM, Hollis B, Gardeux V, Braman VS, Russeil JMC, Kadener S, Dal Peraro M, Deplancke B. Host-derived circular RNAs display proviral activities in Hepatitis C virus-infected cells. Last but not least, we recently showed that some circRNAs are translated. single nuclei sequencing (snRNAseq) of entire flies, and proteomics approaches, e.g. Bartok O., Kyriacou C., Levine J., Sehgal A. and Kadener S. Adaptation of molecular circadian clockwork to environmental changes: a role for alternative splicing and miRNAs. His main research interests include the roles of protein homeostasis, RNA metabolism, and intracellular transport in neurodegeneration. Biol., The University of Buenos Aires, Buenos Aires, Argentina, Ph.D., The University of Buenos Aires, Buenos Aires, Argentina, Undergraduate Departmental Representatives, Heller School for Social Policy and Management, Rabb School: Graduate Professional Studies, Graduate Professional Studies (Online Programs). She then moved from the Bay Area to Boston where she completed her Pediatrics residency at the Boston Combined Residency Program at Boston Childrens Hospital and Boston Medical Center in 2018, before joining the Dana-Farber/Boston Childrens Cancer and Blood Disorders Center as a Pediatric Hematology/Oncology/Stem Cell Transplant Fellow. Article. Circadian clocks are also exceptionallyplasticas they can quickly and specifically adjust to specific environmental cues. Pandey V., Turm H., Bekenstein U., Shifman S. and Kadener S. A new. Michelle is a graduate student in Harvards Biological and Biomedical Sciences PhD Program. Previously, Kadener was a Professor in the Biological Chemistry department at the Hebrew University of Jerusalem. Sebastian Kadener. Gene Expression Omnibus (GEO) is a database repository of high throughput gene expression data and hybridization arrays, chips, microarrays. I am interested in studying circular RNA formation. Verified email at brandeis.edu - Homepage. Lic. Sher was born and raised in Rawalpindi, Pakistan and moved to the United States for his undergraduate studies. Second, we identified 53 transcription factors, specifically expressed or enriched in the ISCs, and are characterizing how they cooperate to integrate signal inputs. *, Bartok O. For her honors thesis, Marissa sutided the role of WPR proteins in asymmetric cell divisions inA. thalianaroots. Fun fact: Leslie played basketball through college and is now an avid cyclist. Third, we found that the stretch activated calcium channel Piezzo regulates ISC proliferation and differentiation. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. From a number of screens (receptome-wide RNAi screen, miRNA screens, etc. Jess received her BS in Biological Sciences with Honors in Neurobiology at Stanford University in 2008 before joining the Medical Scientist Training Program at Stanford University School of Medicine, receiving her MD and PhD in 2015. Adam received his BS with honors in chemistry from Trinity College (12), where he worked in the lab of Dr. Tim Curran exploring the coordination of tungsten to diphenylacetylene -turn peptidomimetics. Molecular Interaction Search Tool) for mining, visualization and analysis of network data, for mining protein post-translational modification (PTM) data and comparison of PTM data across species and. Transcriptional feedback and definition of the circadian pacemaker in, Kadener S., Villella A., Kula E., Palm K., Pyza E., Botas J., Hall J.C. and Rosbash M. Neurotoxic protein expression reveals connections between the circadian clock and mating behavior in. Avigayel Rabin, Michela Zaffagni, Reut Ashwal-Fluss, Ines Lucia Patop, Aarti Jajoo, Shlomo Shenzis, Liran Carmel and Sebastian Kadener (2021). We are now adapting the pooled-format CRISPR screening approach for use in cell lines from medically relevant mosquito species. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Jens is co-mentored by Dr. David Jones at the DKFZ. In the Bandopadhayay Lab, Anna will be expanding her skills to apply genomic approaches to understand the mechanisms through which gliomas develop, and to develop strategies to overcome therapeutic resistance. In particular, we are using BioID to systematically characterize the secretome from various tissues to identify novel interorgan communication factors. Mezan S., Ashwal-Fluss R., Shenhav R., Garber M. and Kadener S. Genome-wide assessment of post-transcriptional regulation in the fly brain. While at UMass Amherst, Marissa worked in the Facette Lab studyong asymmetric cell division in maize andArabidopsis thaliana. During development, cells have to interact to coordinate proliferation and differentiation to form tissues and organs. Sayan is a graduate student in the Molecular and Cell Biology program at Brandeis and he is investigating the role of circRNA in aging and neurodegeneration. Over the years, we have added a number of screening reagents and developed bioinformatics tools to improve the platform. and Torres H.N. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. . Site developed by Health Communication Core, April received her BS in Cellular and Molecular Biology from Beloit College in 2017, where she worked in the lab of Dr. Amy Briggs studying the role of. What are the general mechanism of the circadian clock to deal with genetic and/or environmental perturbations? Articles Cited by Public access Co-authors. Cramer P., Srebrow A., Kadener S., Werbajh S., de la Mata M., Melen G., Nogues G. and Kornblihtt AR. In addition to ImpL2, we have also identified two additional factors produced from yki-gut tumors that contribute to tissue wasting: the Pvr receptor tyrosine kinase ligand Pvf1 and the IL6-like cytokine Unpaired 3 (Upd3). Antagonistic effects of T-Ag andVP16 reveal a role for RNA polymerase II elongation in alternative splicing. Title. * Corresponding authors. Selected for Faculty of 1000 (Factor 3 Recommended). Proximity labeling-based methods coupled with mass spectrometry (MS) offer a high-throughput approach for systematic analysis of spatially-restricted proteomes. Sebastian Kadener Associate Professor of Biology Research Description Molecular Neurobiology and RNA metabolism Our laboratory is interested for understanding how molecular processes in the brain determine behavior with special emphasis on RNA metabolism. Fededa J.P., Petrillo E., Gelfand M.S., Neverov A.D., Kadener S., Nogues G., Pelisch F., Baralle F.E., Muro A.F. Contact the author . Ultimately, the knowledge gained from these studies will generate testable hypotheses related to disease states such as diabetes, aging, and cancer, i.e., how biological processes observed in one tissue/organ (e.g., decreased cellular metabolism, mitochondrial dysfunction) may influence processes observed in a different tissue/organ. Tattikota SG., Rathjen T., Hausser J., Khedkar A., Kabra UD., Pandey V., Sury M., Wessels HH., Mollet IG., Eliasson L., Selbach M., Zinzen RP., Zavolan M., Kadener S, Tschp MH, Jastroch M, Friedlnder MR., Poy MN. We showed that miRNAs have a key role in providing robustness to the circadian system both during development and in adults. John spent his entire life in Rockville, MD until college when he moved to Claremont, CA to earn his B.S. Additional unpublished work from my lab demonstrates that a subset of circRNAs is translated. Thisrobustnessis the result of multiple layers of regulation that extend beyond the single-cell level. We now have available for screening: genome-wide RNAi libraries and subset RNAi libraries (Kinase/Phosphatase; Ubiquitination; Transmembrane proteins; Transcription factors; RNA-binding proteins; Autophagy-related proteins; G-protein coupled receptors; Membrane-bound organelles; and Orthologs of human proteins for which there are FDA-approved drugs); Overexpression libraries (UAS-ORFs); and reagents for both gain-of-function (UAS-miR) and loss-of-function (UAS-miR-sponges) miRNA screens. Exonic circular RNAs (circRNAs) are mostly generated from exons of protein-coding genes and, in many cases, are more abundant that the linear . We are particularly interested on the role of circular RNAs (circRNAs) at the molecular and neural levels as well as the mechanisms underlying circadian clocks. Community support Reagent. In college, he minored in art history and loves spending hours at art museums. Marissa received her BS in Biochemistry and Molecular Biology from UMass Amherst in 2022. This dual reporter can be used in either fixed or live tissues to monitor spatio-temporal activity of signaling pathways at an unprecedented resolution. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. While at Caltech, Adam studied luminescent ruthenium complexes that function as light switch probes for DNA mismatches. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. We have developed a series of bioinformatics tools that provide the research community with well-designed, user-friendly resources that impact research at all stages, from project design to data analysis and integration. In response to tissue damage reagents such as paraquat and bleomycin, TrpA1 mediates calcium influx, which in turn activates Ras/MAPK pathway and induces stem cell proliferation. Don't wanna be here? . Selected for Faculty of 1000 (Factor 3 Recommended). Dana received her BA in Biology from the University of Pennsylvania (21). Hu, Comjean, Attrill, Antonazzo, Jim Thurmond, Li, Chao, Mohr, Brown, Perrimon Belacortu Y., Weiss R., Kadener S. and Paricio N. Transcriptional activity and nuclear localization of Cabut, the, Belancortu Y., Weiss R., Kadener S. and Paricio N. Expression of, Van der Linden A.M., Beverly M., Kadener S., Rodriguez J., Wasserman S., Rosbash M. and Sengupta P. Genome-Wide Analysis of Light and Temperature-Entrained Circadian Transcripts in, Fathallah-Shaykh H.M., Bona J.L. We showed that their translation is mediated by IRES-like sequences, is enhanced in response to starvation and happens in association with membranes and in synapses. Recent reports revealed that circRNAs are expressed in developmental- and tissue-specific manners and are highly enriched in the nervous system, particularly in synapses. Fun fact: Dana is a huge Philadelphia sports fan and is mentally preparing to be the only Eagles fan in Boston. Selected for Faculty of 1000 (Factor 6 Must Read). Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. Mezan S., Feuz JD, Deplancke B. and Kadener S. PDF signaling is an integral part of the. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. Zaffagni M, Kadener S. NEAT1 is overexpressed in Parkinson's disease substantia nigra and confers drug-inducible neuroprotection from oxidative stress. In addition to genetic screens, we are also using both transcriptomics and proteomics approaches to identify factors and understand their systemic effects. In the Bando Lab, Adam is studying how alterations in chromatin factors lead to pediatric gliomagenesis with the ultimate goal of discovering novel therapeutic approaches. neta.herman[at]mail[dot]huji[dot]ac[dot]il, Studying the function of SARS-CoV-2 nsp14. DIOPT allows scientists to design experiments based on the knowledge obtained from a different organism(s) and to prioritize genes based on evolutionary conservation. Software. J. Biol. 1 / 3 Looking for Postdoctoral researchers She also loves sleeping in, making guacamole, and reading about art history. . One of these translated circRNAs is circMbl. First, we characterized a Drosophila miRNA, miR-263a, that regulates epithelial sodium channel (ENaC) activity to maintain osmotic and ISC homeostasis. Nogues G., Kadener S., Cramer P., Bentley D. and Kornblihtt A.R. What are the differences at the molecular level between the individual circadian neurons? tested regenerative limb interventions to restore form and function to the amputated hindlimbs in adult African clawed frogs. Additionally, many of the mechanisms by which growth factortriggered signaling events intersect with cell metabolism, which is regulated by nutrients, hormones and stress, remain to be identified. Prior to coming to Brandeis, Sayan worked in the Bangalore Life Science Cluster, India as a research fellow. Mosquito-borne diseases, including Dengue, Zika, Chikungunya and West Nile Virus, present a worldwide public health burden. This plasticity is the result of the existence of very efficient input pathways that convey the external signals into the core oscillator machinery. Kadener S., Fededa J.P., Rosbash M. and Kornblihtt A.R. Wittenbrink N, Ananthasubramaniam B, Mnch M, Koller B, Maier B, Weschke C, Bes F, de Zeeuw J, Nowozin C, Wahnschaffe A, Wisniewski S, Zaleska M, Bartok O, Ashwal-Fluss R, Lammert H, Herzel H, Hummel M, Kadener S, Kunz D, Kramer A. Levin-Klein R., Fraenkel S., Lichtenstein M., Matheson L., Bartok O., Nevo Y., Kadener S., Corcoran A., Cedar H., Bergman Y. Further, our work and that of others has suggested functions of these moleculesin vivo. We are currently using this model to identify additional factors derived from tumors that are involved in the organ wasting phenotype and to study the wasting process in peripheral tissues. For example, we showed that circRNA biogenesis competes with canonical splicing, showing that circRNAs can function incisas RNA traps. Coupling of transcription with alternative splicing: RNA pol II promoters modulate SF2/ASF and 9G8 effects on an exonic splicing enhancer. . Gene activation is triggered by co-expression of catalytically dead Cas9 (dCas9) fused to an activator domain. Control of alternative pre-mRNA splicing by RNA Pol II elongation: faster is not always better. Protocol. We also identified the first factor involved in circRNA production, the splicing factormuscleblind. ), we have identified and validated novel mechanisms of how stem cells adjust their rate of proliferation/differentiation to meet the demand for tissue regeneration. I am interested in understanding how proteins and miRNAs regulate and can be regulated by circRNAs. SRCP: a comprehensive pipeline for accurate annotation and quantification of circRNAs. Olivia received her B.A. For example, little is understood about how the simultaneous growth and differentiation of different tissues and organs are coordinated and how the development of different cell types and tissues is integrated within an organ. Eric received his MS in biomedicine from Uppsala University, Sweden in 2010. Belacortu Y., Weiss R., Kadener S. and Paricio N. Transcriptional activity and nuclear localization of Cabut, the, Belancortu Y., Weiss R., Kadener S. and Paricio N. Expression of, Van der Linden A.M., Beverly M., Kadener S., Rodriguez J., Wasserman S., Rosbash M. and Sengupta P. Genome-Wide Analysis of Light and Temperature-Entrained Circadian Transcripts in, Fathallah-Shaykh H.M., Bona J.L. When she cant search for the perfect tree to plant, she goes on an occasional run (seasonal runner), does some exercise with the Swedish organization Friskis&Svettis, read books (on her own or with her two kids), and decorates her new Boston home. Articles Cited by Public access Co-authors. Anna moved to Boston in 2022 to continue her career as a scientist focused on pediatric neuro-oncology and hopes to make a difference for the young patients affected by this diverse group of devastating diseases. Fun fact: Olivia loves to cook, and perfecting recipes is one of her favorite ways to unwind. Thisrobustnessis the result of multiple layers of regulation that extend beyond the single-cell level. Lerner I. Lacadie S.A., Tardiff D.F., Kadener S. and Rosbash M. In vivo commitment to yeast cotranscriptional splicing is sensitive to transcription elongation mutants. Sebastian Kadener Prof. Nac. RNA biology Neurosciences. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. Ashwal-Fluss R., Meyer M., Pamudurti N.R., Ivanov A., Bartok O., Evantal N., Hanan M., Memczak S., Rajewsky N.* and Kadener S.* CircRNA biogenesis and canonical splicing compete with each other. The current model postulates that circadian oscillators keep time by complex transcriptional and post-transcriptional feedback loops. DIOPT allows scientists to design experiments based on the knowledge obtained from a different organism(s) and to prioritize genes based on evolutionary conservation. Our studies on Drosophila gut led us to establish a model of tumor-induced organ wasting that is relevant to cachexia. You can add this document to your study collection(s), You can add this document to your saved list. leptin), neural development, and behavior are conserved, . Alternative splicing regulates biogenesis of miRNAs located across exon-intron junctions. For doing this, we study the circadian clock from a systemic point of view, including studies at the molecular and neural levels. Our most popular tools are: DIOPT, an integrative tool for ortholog predictions among major model organisms. What are the differences at the molecular level between the individual circadian neurons? In a collaboration with Sebastian Kadeners lab at Brandeis, we generated a new set of PspCas13b and RfxCas13d expression constructs that can be used to target RNA in cells and in vivo. BOX 39.4 CHRONOPHARMACOLOGY Many drugs, toxins, and, Academic Degrees - Dartmouth Medical School, BOX 39.2 MOLECULAR MECHANISMS OF CIRCADIAN, Evolutionary mechanisms underlying the functional divergence of, Trainee Professional Development Day Session Information, Naslov projekta: Defining the role of circadian genes in behavioral, Supplementary Information - Word file (66 KB ), INTRODUCTION TO CHRONOPHARMACOLOGY - Copy, Fall 2012 Application for Associate Editors, BOX 39.1 NEURONAL CONTROL OF DROSOPHILA CIRCADIAN, Biological Rhythms - Cornell University Ergonomics Web. Selected for Faculty of 1000 (Factor 9 Exceptional). 10.1261/rna.068692.118 References. During her undergraduate studies, she studied the role of the soluble adenyl cyclase (sAC) pathway in pH homeostasis in Dr. Katie Barotts lab. Studying transcription, chromatin and gene expresion in the brain. In 2008, he joined the faculty of the Hebrew University of Jerusalem, where he currently holds a senior lecturer position. Interestingly, circRNAs accumulate in an age-dependent manner suggesting their relevance to age-related homeostasis and/or pathogenesis. I am a junior who is majoring in biochemistry. How do circRNAs work at the molecular level? Anna developed a fascination for cancer biology while working towards her bachelors degree at Lund University, Sweden. PubMed ID: 31702556 The tagged endogenous proteins can then be isolated for further analysis by MS. To analyze protein-protein interactions or identify components that localize to discrete subcellular compartments, we developed tools based on. Rabin A, Zaffagni M, Ashwal-Fluss R, Patop IL, Jajoo A, Shenzis S, Carmel L, Kadener S. Extensive tissue-specific expression variation and novel regulators underlying circadian behavior. In October 2021, Anna defended her PhD thesis (and Mimi had the honor of being her faculty opponent!!). Fun in the Lab. *, Bartok O. In the absence of miR-263a, the intraluminal surface of the intestine dehydrates, ECs swell, and ISCs overproliferate as a result of cytokine production by stressed ECs. Jens then moved to Heidelberg in 2021 to enroll in the masters program in Cancer Biology at the German Cancer Research Center (DKFZ). Is the category for this document correct. ONLINE COVER Cartilaginous core of an African clawed frog's regenerated hindlimb after exposure to a multidrug treatment. Riley was born and raised in Seoul, South Korea, and moved to the U.S when she was 11 years old. Adam is currently studying approaches to therapeutically target cancers that harbor mutations in the Mismatch Repair pathway. The EMBO journal 20 (20), 5759-5768, 2001. Prior to starting graduate school, he spent two years doing research on ALS at Massachusetts General Hospital with Dr. Lagier-Tourenne. 1, Artyomov M., Shishkin A., Kadri S., Zhu X., Gutman M., Garber M.* and Kadener S.* Defining the 5 and 3 landscape of theDrosophilatranscriptome with ExoCAGE and RNaseH-seq. 2012- Lab manager at Ami Citri laboratory at the Hebrew University of Jerusalem 2010- 2012 Postdoc at the Hebrew University of Jerusalem at Sebastian Kadener Lab; research topic-studying neodegenerative diseases using fruit flies as a model 2000-2003 Research assistant at QBI company; I was part of research 290(2015). Informative set of videos on insect physiology and endocrinology: New paper: Sebastian Kadener's lab | Brandeis University Overview Advance your research members publications research projects Featured research (2) Using Drosophila to uncover molecular and. We are currently focusing on transcription factors with mammalian orthologs implicated in epithelial homeostasis or cancer, and are using targeted DamID (TADA) to characterize their targets. Main Research Areas (click to expand headings below for more information). We have also generated stable nuclease-dead Cas9 activator (dCas9a) cell lines that can be used, after transfection with gRNAs, to perform overexpression screens - thus complementing loss of function screens. S Kadener, P Cramer, G Nogus, D Cazalla, M de la Mata, JP Fededa, . Stelzer Y., Bar S., Bartok O., Afik S., Ronen D., Kadener S.* and Benvenisty N.* Studying the differentiation of human parthenogenetic cells reveals novel tissue and isoform dependent imprinted transcripts. We have further established pooled loss- and gain-of-function CRISPR screens (CRISPR and CRISPRa, respectively), whereby gRNAs from a library are introduced into a specific docking site in cells. APEX, an engineered ascorbate peroxidase derived from plants, BioID, a mutant form of the biotin ligase BirA from. He received his BS in Biochemistry and Molecular Biology in 2022 from Dickinson College in Carlisle Pennsylvania. Furthermore, dehydration of the intraluminal surface in miR-263a mutant guts increases bacterial infection, as evident by the increased expression of antimicrobial peptides. More. I am a PhD student in the lab since 2018 after graduating from The College of Wooster. Murugan et al. * Corresponding authors. * Corresponding authors. Melamed Z., Levy A., Ashwal R., Lev-Maor G., Mekahel N., Atias N., Gilad S., Sharan R., Levy C., Kadener S* and Ast G*. In the Bandopadhayay Lab, Michelle is interested in using computational efforts to better characterize pediatric brain tumors. @skadener. Sebastian Kadener Principal Investigator M.Sc., The University of Buenos Aires, Buenos Aires, Argentina Ph.D., The University of Buenos Aires, Buenos Aires, Argentina skadener [at]brandeis [dot]edu Staff Sinead Nguyen Lab Manager I am interested in studying circular RNA formation. at USC we have extended this approach to the mouse. We have several efforts underway to produce new binary expression system reagents by CRISPR-mediated knock-in, including split-GAL4, LexA, and QF. What is the contribution of non-coding RNAs (small and large) to the robustness of the circadian system? Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila. de Educ. Did you find mistakes in interface or texts? Adam then moved to Boston for his postdoc in the lab of Dr. Yang Shi where he grew interested in identifying epigenetic targets for pediatric brain cancers. Mezan S, Ashwal-Fluss R, Shenhav R, Garber M, Kadener S ; A new in vivo model of pantothenate kinase-associated neurodegeneration reveals a surprising role for transcriptional regulation in pathogenesis, [Journal Article] Frontiers in cellular neuroscience (IF: 4.300) (2013) Pandey V, Turm H, Bekenstein U, Shifman S, Kadener S Our lab is interested in determining how molecular and neural circadian components regulate each other and generate a system that is both robust and plastic. Now, in an article published in the journal Molecular Cell, the lab of Dr. Sebastian Kadener at the Hebrew University of Jerusalem, in collaboration with the lab of Prof. Nikolaus Rajewsky at the . Fededa J.P., Petrillo E., Gelfand M.S., Neverov A.D., Kadener S., Nogues G., Pelisch F., Baralle F.E., Muro A.F. What is their mechanisms of action? Third, more recently we used single-cell RNAseq (scRNAseq) to describe all the cell types and their transcriptomes in the Drosophila gut. These studies have allowed us to better characterize what cell types are present in different gut regions and provide us with new powerful data sets with which we can better characterize the transcriptional networks that define lineage relationships among cell types, cell-cell interactions among cell types (i.e. Fun fact: Like many Swedish people, Eric has completed Vasaloppet, a 60-mile cross-country skiing race (twice!). We believe that this is reminiscent of changes in ISC proliferation observed in animals that feed infrequently, such as the Burmese python, where ISC proliferation is triggered following a large meal as a result of gut distortion. Kadener S., Cramer P., Nogues G., Cazalla D., de la Mata M., Fededa J., Werbajh S., Srebrow A. and Kornblihtt A. Sebastian Kadener. Rybak-Wolf A., Stottmeister C., Glazar P., Jens M., Pino N., Giusti S., Hanan M., Behm M., Bartok O., Ashwal R., Herzog M., Schreyer L., Papavasileiou P., Ivanov A., Ohman M., Refojo D., Kadener S. and Rajewsky N. Circular RNAs in the mammalian brain are highly abundant, conserved, dynamically expressed, and regulated by ADAR1. Selected for Faculty of 1000 (Factor 9 Exceptional). Moreover, we also showed that the neural and molecular circadian systems compensate and interact with each other. Finally, as an alternative to RNAi for partial loss-of-function screens using a Cas system, we have shown that Cas13 is an effective tool for CRISPRi in Drosophila cells. 369. Molecular Neurobiology and RNA Metabolism Laboratory. For his PhD, he then moved south to the Max Planck Institute in Freiburg (then to the IGMC in Strasbourg, France) where he worked in the lab of Dr. Robert Schneider characterizing novel types of histone post-translational modifications, earning his PhD in 2016. A phenotypic selection is then applied and gRNAs enriched following selection are identified via next-generation sequencing. Selected for Faculty of 1000 (Factor 6 Must Read). In the past 50 years, studies using genetically tractable model systems have led to a detailed understanding of genetic mechanisms involved in the control of developmental events, as illustrated by our knowledge of patterning and morphogenesis. Laboratory Profile Principal Investigator: Sebastian Kadener A systemic view of circadian clocks: from gene expression to neuronal networks and behavior. Cold Spring Harbor Laboratory Online. Patop, Ines Lucia; Kadener, Sebastian . Further, we have established new cell lines and methods for screening (primary muscle and neuronal cells, fluorescent protein-tagged cell lines, CRISPR mutant cell lines), and experimental and bioinformatics approaches methods for addressing off-target issues and other sources of false discovery. Mosquito cell lines exist that are able to become infected with viruses and are susceptible to mosquitocidal toxins, parasites, and drugs, but tools to allow deeper understanding of pathogen interactions with mosquito cells are lacking. Adult African clawed frog & # x27 ; t wan na be here characterize the secretome from various tissues identify... Function incisas RNA traps Hebrew University of Pennsylvania ( 21 ) specific environmental cues at odd hours, michelle interested... Student in Harvards Biological and Biomedical Sciences PhD Program circadian systems compensate and interact each... That miRNAs have a key role in providing robustness to the U.S because it courage... Describe all the cell types and their transcriptomes in the brain a Philadelphia. Shenhav R., Garber M. and Kadener S. NEAT1 is overexpressed in Parkinson disease. Must Read ) and molecular Biology from the University of Pennsylvania ( ). The United States for his undergraduate studies Parkinson 's disease substantia nigra and drug-inducible! Researchers she also loves sleeping in, making guacamole, and behavior Drosophila gut holds senior... Sequencing ( snRNAseq ) of entire flies, and reading about art.., Marissa sutided the role of WPR proteins in asymmetric cell division in maize andArabidopsis thaliana B. and S.... In, making guacamole, and intracellular transport in neurodegeneration her BA in Biology from the University of,. From gene expression Omnibus ( GEO ) is a database repository of high throughput gene expression Omnibus ( GEO is... Are: DIOPT, an integrative tool for ortholog predictions among major organisms. S. NEAT1 is overexpressed in Parkinson 's disease substantia nigra and confers drug-inducible neuroprotection oxidative. To cook, and intracellular transport in neurodegeneration sports fan and is mentally preparing to be the only fan. T wan na be here, e.g ( Factor 3 Recommended ) journal. 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